The utility model relates to a check valve of a pressurizing and water filling pump, which is characterized in that the check valve of a pressurizing and water filling pump is composed of an upper valve body 7 which is connected with an outlet of a pressurizing electric submersible pump, a lower valve body 1 which is connected with an oil pipe, a flow guide frame 2, a valve rod 3, a spring 4, a valve seat 5 and a valve core 6, the upper valve body 7 is provided with the lower valve body 1 into a whole in a sealing match mode, the flow guide frame 2 is pressed and arranged on the lower valve body 1, the valve seat 5 is pressed and arranged on the upper valve body 7, the valve seat 5 and the valve core 6 mutually form the match surface of a conical surface, one end of the valve rod 3 is connected with the valve core 6, the spring 4 is sheathed on the valve rod and the other end extends into the flow guide frame 2. The utility model has the advantages that the mounting operation is fast and convenient; the structure design of the check valve of a pressurizing and water filling pump ensures sufficient over flow area, and quickly restores close state, so that the water filling effect of the pressurizing electric submersible pump is ensured; the application range is broad, and the check valve of a pressurizing and water filling pump has good commercial development prospect.