基本信息:
- 专利标题： 一种基于供体与受体的嵌合体的嵌合率的检测方法
- 专利标题（英）：Detection method of chimeric rate of chimera based on donor and receptor
- 申请号：CN201910655106.8 申请日：2019-07-19
- 公开(公告)号：CN110551804A 公开(公告)日：2019-12-10
- 发明人： 彭海
- 申请人： 武汉拜肯生物科技有限公司 , 彭海
- 申请人地址： 湖北省武汉市洪山区街道口南路6号京韵花园1-B-401
- 专利权人： 武汉拜肯生物科技有限公司,彭海
- 当前专利权人： 武汉拜肯生物科技有限公司,彭海
- 当前专利权人地址： 湖北省武汉市洪山区街道口南路6号京韵花园1-B-401
- 代理机构： 南京中高专利代理有限公司
- 代理人： 祝进
- 主分类号： C12Q1/6869
- IPC分类号： C12Q1/6869 ; G16B20/00
The invention relates to a detection method of the chimeric rate of a chimera based on a donor and a receptor. The detection method includes the steps that a multi-PCR amplification product is obtained through detection sites of genomic DNA of the multi-PCR amplification chimera by utilizing a primer group; the multi-PCR amplification product is subjected to high-throughput sequencing to obtain sequencing fragments; allelotypes of true polynucleotide polymorphism of the donor, the receptor and the chimera of the detection sites are obtained according to the sequencing fragments; whether the chimera is contaminated or not is judged by utilizing the allelotypes of the true polynucleotide polymorphism; and if the chimera is not contaminated, the chimeric rate of the chimera is calculated. Theprovided detection method of the chimeric rate of the chimera is high in sensitivity and accurate.
公开/授权文献:
- CN110551804B 一种基于供体与受体的嵌合体的嵌合率的检测方法 公开/授权日：2023-01-17