基本信息:
- 专利标题： 一种以人Ad5复制缺陷型腺病毒为载体的寨卡病毒病疫苗
- 专利标题（英）：Zika virus disease vaccine taking human Ad5 replication-defective adenovirus as vector
- 申请号：CN201710375601.4 申请日：2017-05-24
- 公开(公告)号：CN107190013A 公开(公告)日：2017-09-22
- 发明人： 陈薇 , 郭强 , 侯利华 , 吴诗坡 , 宋小红 , 付玲 , 王步森 , 张金龙
- 申请人： 中国人民解放军军事医学科学院生物工程研究所
- 申请人地址： 北京市丰台区东大街20号
- 专利权人： 中国人民解放军军事医学科学院生物工程研究所
- 当前专利权人： 中国人民解放军军事医学科学院生物工程研究所
- 当前专利权人地址： 北京市丰台区东大街20号
- 代理机构： 北京市众天律师事务所
- 代理人： 李新军
- 主分类号： C12N15/40
- IPC分类号： C12N15/40 ; C12N15/861 ; C12N7/01 ; A61K39/12 ; A61P31/14
The invention discloses a codon-optimized nucleotide sequence capable of expressing an Env protein of a zika virus. The sequence can be fused with a protein prM and a protein prM endogenous signal peptide of a codon-optimized zika virus; after the sequence is inserted into a shuttle vector pDC316, the sequence and an auxiliary vector pBHGlox_E1, 3Cre realize cotransfection of a cell HEK293, so as to package an E1 and E3 combined missing-recombinant adenovirus taking a replication-defective human type-5 adenovirus as a vector; the recombinant adenovirus vector can efficiently express an envelope protein of the zika virus in an infected cell. After the nucleotide sequence-inserted recombinant adenovirus serving as a vaccine is immune to an animal for single time, strong humoral immune and cellular immune responses can be quickly induced. A zika vaccine taking the recombinant adenovirus as the vector is suitable for large-scale and rapid preparation and can be used for emergent vaccination for a large scale of people in a zika outbreak and prophylactic immunization for people at ordinary times.
公开/授权文献:
- CN107190013B 一种以人Ad5复制缺陷型腺病毒为载体的寨卡病毒病疫苗 公开/授权日：2020-02-14