The invention discloses a traditional Chinese medicinal composition containing in-vitro cultured calculus bovis and having the efficacies of clearing away heat and purging pathogenic fire as well as dispelling wind and relieving pain, belonging to the field of traditional Chinese medicines. The traditional Chinese medicinal composition is prepared from the following medicinal materials by weight: 0-1.8 g of calculus bovis factitius, 0.2-2 g of in-vitro cultured calculus bovis, 30g of herba menthae, 40g of flos chrysanthemi, 16g of schizonepeta spikes, 16g of radix angelicae, 16g of rhizoma ligustici wallichii, 50g of fructus gardeniae, 16g of rhizoma coptidis, 10g of cortex phellodendri, 50g of radix scutellariae, 80g of radix et rhizoma rhei, 50g of fructus forsythiae, 16g of radix paeoniae rubra, 50g of radix angelica sinensis, 64g of radix rehmanniae, 16g of radix platycodi, 10g of licorice root, 80g of gypsum, and 10g of borneol. For the traditional Chinese medicinal composition, in-vitro cultured calculus bovis is adopted for replacing calculus bovis factitius in the prescription, so that the efficacies are improved, meanwhile, the side effects are reduced, moreover, the quality is controllable, the potential safety hazards are small, and the traditional Chinese medicinal composition has the obvious economic benefits and social benefits.