The invention discloses an automatic vertical bottle feeding device which comprises a working frame, a conveying line, an interval separating mechanism, an overturning mechanism, a guide tube and a pushing mechanism, wherein the conveying line is arranged on the working frame; the interval separating mechanism is arranged on the conveying line and is used for rowing up and vertically and horizontally separating packaging bottles at intervals; the overturning mechanism comprises an overturning plate and is arranged in front of the tail end of the conveying line, the overturning plate is mounted between two overturning supports and is driven by a first overturning device to rotate, a plurality of round holes are formed in the overturning plate, the positions of the round holes are opposite to the interval separating mechanism on the conveying line, and the diameter of each round hole is larger than that of a bottle cover of each packaging bottle and less smaller that of the bottle bottom of the packaging bottle; the guide tube is arranged under the overturning mechanism, a plurality of channels are formed in the guide tube, and the positions of the channels are opposite to those of the round holes; the pushing mechanism is arranged under the guide tube and is used for pushing the packaging bottles. The automatic vertical bottle feeding device disclosed by the invention is simple and practical in structure and can automatically and vertically arrange the packaging bottles neatly and orderly, so that a good preparation is made for a next box packaging procedure.