The invention discloses an animal protein pet chew. The chew is made from animal protein materials, with different components and by a certain process. The chew is good for massaging teeth and cleaning an oral cavity, is rich in vitamin A, vitamin D, vitamin B1, vitamin B6, vitamin B12, vitamin K and concentrated minerals, has completely balanced nutrition and helps promote health of intestines of pet dogs and decrease stink of excrement. Collagen is effective in enhancing physique of dogs, enhancing immunity of the dogs, improving bone elasticity and toughness of the dogs and improving motor function of the dogs, plays an important role in keeping skin and fur of the pet dogs healthy and bright and is effective in preventing occurrence of skin diseases. The chew is suitable for dogs growing and developing in different sizes and functions in adjusting appetite. Additionally, the invention further discloses a preparation method of the chew.