The invention discloses a starting speed-regulation method and device of an electric bicycle. The starting speed-regulation method of the electric bicycle comprises the steps that output voltage of a handlebar of the electric bicycle is detected; the loop current threshold value of the electric bicycle is determined according to the output voltage; the real-time loop current of the electric bicycle is detected and compared with the loop current threshold value; the PWM value of control signals is regulated according to the comparison result; the motor regulating driving force of the electric bicycle is controlled according to the PWM value of the control signals, and the driving force is used for changing the acceleration of the electric bicycle. According to the starting speed-regulation method and device of the electric bicycle, the driving force on wheels by a motor is regulated according to the output voltage of the handlebar and the loop current between a controller and the motor. Compared with a traditional speed regulation method, flexible regulation of the driving force under different output voltages is achieved, and therefore the phenomenon of onrush occurring at the moment of starting of the electric bicycle is avoided.